Coding

Part:BBa_K4170003:Design

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-27)


LbuCas13a assembly part 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI.rc site found at 728


Design Notes

This part derives from pGJK_His-SUMO-LbuCas13a plasmid (Plasmid #172488) by mutagenesis PCR to efficiently mutate illegal restriction sites. In addition, the primers exploited incorporate BsaI recognition sites external of the amplified sequence to allow for efficient Golden Gate assembly with the parts LbuCas13a assembly parts 1,3 and 4 along with the linearilized plasmid backbone


Source

This part derives from pGJK_His-SUMO-LbuCas13a plasmid (Plasmid #172488).

References